One Step COVID-19 Antigen Rapid Detection Kit aims to expedite the screening process by providing rapid differential diagnosis of COVID-19 virus infection.
The kit is based on immunochromatographic assay for the detection of novel Coronavirus antigen (viral nucleoprotein) from nasal swab, nasopharyngeal swab, endotracheal aspirate or bronchoalveolar lavage obtained from a patient with signs and symptoms of respiratory infection.
Albot-Lorven One-Step COVID-19 Antigen test is an antigen-capture immunochromatographic assay, detecting the presence of COVID-19 viral nucleoprotein antigen in nasal and nasopharyngeal swab samples.
The assay involves extraction of viral antigen from the sample or specimen, followed by solid-phase immunoassay technology for the detection of extracted antigen.
With the help of colloidal gold COVID-19 antibodies are first conjugated. The conjugated antibodies are then deposited on the conjugate pad and immobilized on the test zone of the nitrocellulose membrane. The gold-antibody conjugate is rehydrated when a specimen is added.
If there are any COVID-19 antigen in the specimen it will interact with the gold conjugated antibodies. The antigen-antibody-gold complex will migrate towards the test window of the test zone where they will be captured by immobilized antibodies, forming a visible pink line (test band) indicating a positive result. There will be no pink line in the test zone (T), if COVID-19 antigen is absent in the specimen.
To serve as internal process control, a control region was designed to indicate the test performance. By utilizing the different antigen/antibody reactions, the control line should always be seen after the test is completed. The absence of a pink control line in the control region is an indication of an invalid result.
For proper detection of COVID-19 antigen, correct specimen collection and preparation methods must be followed.
The correct method of collecting samples for the test is:
Nasal Swab Sample –
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